Quick Overview for Biotin Protein (Glucose Oxidase) (ABIN964126)
抗原
宿主
资源
应用范围
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标记
- This Biotin protein is labelled with Glucose Oxidase.
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Supplier Product No.
- b000-07
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Supplier
- Rockland
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原理
- Biotin Glucose Oxidase Conjugated
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纯化方法
- Biotin Glucose Oxidase Conjugated was prepared from chromatographically purified biotin. Biotin Glucose Oxidase Conjugated assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.
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过滤
- Sterile filtered
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Biotin protein (Alkaline Phosphatase (AP))
宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
Biotin protein (HRP)宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
Biotin protein (beta-Gal)宿主: 化学剂 宿主: 合成 DB, ELISA, IHC, WB
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应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Biotin Glucose Oxidase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.
Western Blot Dilution: 1:500 - 1:2,500
ELISA Dilution: 1:8,000 - 1:32,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent) (in 5.0 mL)
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浓度
- 1.0 mg/mL
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缓冲液
- 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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注意事项
- Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Biotin Glucose Oxidase Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Biotin
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物质类
- Chemical
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背景
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Synonyms: Biotin Glucose Oxidase Conjugation, Glucose Oxidase Conjugated Biotin, Biotin conjugated Glucose Oxidase
Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav
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